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TelN Protelomerase from E. coli bacteriophage N15Êprocesses circular and linear plasmid DNA containing the target siteÊtelRLÊto produce linear double-stranded DNA with covalently closed ends. The 56-bpÊtelRLÊtarget site consists of a centralÊtelOÊ palindrome of 22 bp and two 14-bp flanking sequences comprising inverted repeats.ÊtelOÊis separated from these repeats by 3 bp on each side. TheÊtelRLÊsequence is sufficient for TelN-mediated processing. TelN has cutting-ligating enzyme activity and covalently ligates at the cleavage site forming hairpin termini.
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